CultiBag RM. Disposable CultiBags
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- Harriet Elliott
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1 ultiag RM Sensors Single-use sensors for p and O come preinstalled and pre-sterilized with the bag. This avoids risky insertion of traditional sensors. isposable ultiags n optical fibre connects to the sensor patch through a sterile barrier at the end of a sleeve in the bag. Sterility is maintained at all times. The optical fibre transmits light of specific wavelength to the sensor patch and returns the luminescence response from the sensor back to the measuring amplifier. alibration is fast and easy. Operating Volumes escription The ultiag RM is a disposable bag optimised for cell cultivation. ags are available in multiple sizes and configurations depending on customer requirements. The inner layer of the disposable bioreactor bag is made of ethyl vinyl acetate (V), which shows an excellent biocompatibility. pplications Our systems use rocking motion mixing technology which is ideal for cell cultivation with low shear stress. asy to use, it is hassle free and applicable to all cell types, including mammalian cells, stem cells, plant cells, insect cells and microbial cells. ost Reduction and Risk Minimization isposable ags and single-use systems in general used in biopharmaceutical manu - facturing improve process safety and reduce costs at the same time. Time and capacity consuming P & SP operations are minimized. lexibility The ultiag RM is available in 1 L, 2 L, 10 L, 20 L, 50 L, 100 L, 200 L and 600 L bag sizes. ags are available as basic, optical (with p/o sensors) and perfusion pro (with p/o sensors and internal perfusion membrane). dditionally, basic bags with screw caps are also available. asy mplementation, lexible ombinations ultiag RM ags are supplied sterilized and ready to use. Setup times are kept to a minimum. The ultiag RM can be mounted on the bagholder of the rocker easily and is secured on both sides through fixation clamps. Your process can be started right away. Media can be filled into the bag up to the required volume. seed culture is added to the bag and cultivation is started under optimized conditions of aeration, temperature and mixing. ombining your basic rocker with our OSTT RM ontrol Tower will optimize your process for real culturing convenience. Sterile connection and disconnection devices like the iowelder or the iosealer, which are also provided by Sartorius Stedim iotech, can be used to make safe connections between the -lex 374 thermoplastic tubings which are used on all ultiag RM bags. Needle-free sampling ports allow easy and convenient sampling without the risk of cross contamination. ag Size Min [l] Max [l] Total [l] 1 L L 0.2* L 1.0* L 2.0* L 5.0* L 10* L 20* L 60* * ags with sensors might require higher min. working volumes
2 Validation and xtractables Testing ultiag RM ags have been qualified applying the most complex and innovative test regimes. iological, chemical and physical tests combined with extractable testing proove lowest extractables and leechables levels and excellent compatibilty to the relevant pharmacopoeias and guidelines. or more information, please refer to our Validation uide and xtractables uide. leachables testing service is also available. Please contact your local Sartorius Stedim iotech representative for further information. Quality ssurance ll relevant materials are selected following applicable regulations and standards such as, R s, cmps and inhouse guidelines. This includes the terms of delivery and acceptance of our purchasing department. inished ultiag RM bags undergo final product quality control which is certified with the Quality ssurance certificate included with every bag. Quality Management Systems Sartorius Stedim iotech has implemented a certified Quality Management System according to well established standards. The complete Quality Systems ertificates are continuosly updated and can be downloaded on our website: Rapid Supply ll standard ultiag RM ags are available from stock. Multiple warehouse locations ensure a fast delivery all over the world. ag ultiag RM basic: ultiag RM basic screw cap: ultiag RM optical: ultiag RM perfusion pro: ir nlet ir Outlet filter Sample port ill drain and spare ports emale luer, male luer, female MP or male MP connectors with dip tubes Tubing material: -lex 374; Silicone ir nlet ir Outlet filter Sample port ill drain and spare ports emale luer, male luer, female MP or male MP connectors with dip tubes Tubing material: -lex 374; Silicone 38 mm screw cap ir nlet ir Outlet filter Sample port ill drain and spare ports emale luer, male luer, female MP or male MP connectors with dip tubes Optical chemical O sensor Optical chemical Tubing material: -lex 374; Silicone ir nlet ir Outlet filter Sample port ill drain and spare ports emale luer, male luer, female MP or male MP connectors with dip tubes Optical chemical O sensor Optical chemical eed harvest ports cid base ports Tubing material: -lex 374; Silicone; PharMed
3 Ordering nformation 002 L isposable ags Package Type = asic O = Optical P = Perfusion = ustomized Volume 001 = = = = = = = = 600 Units L = Liter ag Version = 1.2 μm pore size (perfusion pro bag) or standard (basic and optical bag) 2 = ag design fits Rocker 2 5 = 5 μm pore size (perfusion pro bag) Vent ilter Type Membrane SM = Sartorius Stedim iotech perfusion pro membrane esign number esign variation Order ode escription asic ags xample: 020L ultiag RM 20 L basic for Rocker L2 ultiag RM 2 L basic for Rocker 2 Optical ags or xample: O020L ultiag RM 20 L optical Perfusion Pro ags xample: P020L--SM ultiag RM 20 L perfusion pro; 1.2 μm pore size P020L5-SM ultiag RM 20 L perfusion pro; 5 μm pore size ustomized ags ustomer own bag design or xample: 002L----01xx ultiag RM 2 L customized customer version
4 ag onfigurations ultiag RM 1 L basic ultiag RM 1L asic (for Rocker 2) 001L 001L2 ip Tube Silicone, -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Port Silicone tubing 3/16" + 5/16" (50 mm), M-Luer ultiag RM 2L asic ultiag RM 2L asic (for Rocker 2) 002L 002L2 ip Tube Silicone, -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer ; Silicone tubing 3/16" + 5/16" (50 mm), M-Luer ultiag RM 10L asic ultiag RM 10L asic (for Rocker 2) 010L 010L2* ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-luer Port Silicone tubing 3/16" + 5/16" (50 mm), M-Luer ultiag RM 20 L basic 020L ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-luer Port ; Silicone tubing 3/16" + 5/16" (50 mm), M-Luer * or bags that fit on the Rocker 2 the ports and are mounted vice versa.
5 ultiag RM 50L basic 050L ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Port ; Silicone tubing 3/16" + 5/16" (50 mm), M-Luer -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP J ultiag RM 100 L basic 100L ; Silicone tubing 3/16" + 5/16" (50 mm) -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Port ; -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer as Outlet ilter, M-Luer ; as nlet ilter 3/16" ose barb Port J ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP J ultiag RM 200 L basic 200L ; Silicone tubing 3/16" + 5/16" (50 mm) -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Port ; -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer as Outlet ilter, M-Luer ; as nlet ilter 3/16" ose barb Port J ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP
6 ultiag RM 2L asic Screw ap 002L----01S ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP ; Silicone tubing 3/16" + 5/16" (50 mm), M-Luerr Screw ap 38 mm ultiag RM 10L asic Screw ap 010L----01S ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Port Silicone tubing 3/16" + 5/16" (50 mm), M-Luer Screw ap 38 mm ultiag RM 20L asic Screw ap 020L----01S ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP Port ; -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Silicone tubing 3/16" + 5/16" (50 mm), M-Luer Screw ap 38 mm
7 ultiag RM 2L Optical O002L ip Tube Silicone, -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer ; Silicone tubing 3/16" + 5/16" (50 mm), M-Luer O sensor ultiag RM 10L Optical O010L ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Port Silicone tubing 3/16" + 5/16" (50 mm), M-Luer O sensor ultiag RM 20L Optical O020L ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer Port O sensor Silicone tubing 3/16" + 5/16" (50 mm), M-Luer
8 ultiag RM 50L Optical O050L ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8"+ 1/4" (1000 mm), M-Luer Port O sensor Silicone tubing 3/16" + 5/16" (50 mm), M-Luer -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP K J ultiag RM 100L Optical O100L -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Silicone tubing 3/16" + 5/16" (50 mm), M-Luer ; -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer O sensor as Outlet ilter, M-Luer ; J Port K ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP K J ultiag RM 200L Optical O200L -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Silicone tubing 3/16" + 5/16" (50 mm), M-Luer ; -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer O sensor as Outlet ilter, M-Luer ; J Port K ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP
9 ultiag RM 600L Optical ; Port Port J Port K; L; M Port N; O Port P Port Q ip Tube Silicone, -lex 374 tubing 3/8" x 5/8" (2000 mm), PharMed tubing 3/8" + 5/8" (600 mm), M-MP O sensor ip Tube Silicone, -lex 374 tubing 3/8" x 5/8" (1000 mm), M-MP Silicone tubing 3/16" + 5/16" (50 mm), M-Luer -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (2000 mm), M-MP as Outlet ilter, M-Luer ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (2500 mm), M-MP ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (2500 mm), M-MP P Q L M Product Label O N K J Label O Label p
10 ultiag RM 2L Perfusion Pro P002L--SM P002L5-SM Port ip Tube Silicone, -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/16" + 3/16" (300 mm), M-Luer (Perfusion eed) -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/16" + 3/16" (300 mm), M-Luer (Perfusion arvest) O sensor ultiag RM 10L Perfusion Pro P010L--SM P010L5-SM Port Silicone tubing 3/16" + 5/16" (50 mm), M-Luer -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/16" x 3/16" (300 mm), M-Luer (Perfusion eed) -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/16" + 3/16" (300 mm), M-Luer (Perfusion arvest) O sensor ultiag RM 20L Perfusion Pro ip tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP P020L--SM P020L5-SM ip Tube Silicone, -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-luer -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/8" + 1/4" (300 mm), M-Luer (Perfusion eed) Port O sensor -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/8" + 1/4" (300 mm), M-Luer (Perfusion arvest)
11 ultiag RM 50L Perfusion Pro J P050L--SM P050L5-SM ip Tube Silicone; -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/8"+ 1/4" (300 mm), M-Luer (Perfusion eed) Port O sensor -lex 374 tubing 1/8" + 1/4" (1000 mm), PharMed 1/8" + 1/4" (300 mm), M-Luer (Perfusion arvest) -lex 374 tubing 1/4" + 7/16" (1000 mm), M-MP Port J L J K ultiag RM 100L Perfusion Pro P100L--SM P100L5-SM -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/4" + 7/16" (1000 mm), PharMed 3/16" x 5/16" (300 mm), M-Luer (Perfusion eed) -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Port ; -lex 374 tubing 1/4" + 7/16" (1000 mm), PharMed 3/16" x 5/16" (300 mm), M-Luer (Perfusion arvest) O sensor as Outlet ilter, M-Luer Port J; K Port L ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP L J K ultiag RM 200L Perfusion Pro P200L--SM P200L5-SM -lex 374 tubing 1/8" + 1/4" (1000 mm), M-Luer -lex 374 tubing 1/4" + 7/16" (1000 mm), PharMed 3/16" x 5/16" (300 mm), M-Luer (Perfusion eed) -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP Port ; -lex 374 tubing 1/4" + 7/16" (1000 mm), PharMed 3/16" x 5/16" (300 mm), M-Luer (Perfusion arvest) O sensor as Outlet ilter, M-Luer Port J;K Port L ip Tube Silicone, -lex 374 tubing 3/8" + 5/8" (1000 mm), M-MP
12 ag ccessories Order ode S S S-----RM S200L-RM S V P P P P P P escription Light onductor ables for ultiag RM optical and perfusion pro (2 pcs) lamps for Light onductor able fixation (2 pcs) ilter eater for outlet filter of ultiag RM 1 L 50 L ilter eater for outlet filter of ultiag RM 100 L 200 L heck Valves for outlet filter of ultiag RM (50 pcs) MP 3/8 P emale ody (40 pcs) MP 1/4 P emale ody (40 pcs) MP 3/8 P/Silicone Male nsert (40 pcs) MP 1/4 P/Silicone Male nsert (40 pcs) MP P/Silicone Male Sealing Plug (40 pcs) MP P emale Sealing ap (40 pcs) Luer conncetors are available on request Sartorius Stedim iotech mb ugust-spindler-strasse oettingen, ermany Phone ax US Toll-ree UK rance taly Spain Japan Specifications subject to change without notice. Printed and copyrighted by Sartorius Stedim iotech mb W Publication No.: ST2002-e10079 Order No.: Ver
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