Model : LAC-60 Please read this manual carefully before using the instrument Labnics Equipment
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1 Autclave / Sterilizer Instructin Manual Mdel : LAC-60 Please read this manual carefully befre using the instrument Labnics Equipment
2 Table f Cntent CHAPTER CONTENT PAGE NO. 1. Befre Use 1 2. Safety Precautin Safety Pwer cnnectin Installatin peratin Maintenance 5 3. Feature and Advantage 5 4. Specificatins 7 5. Parts and Functins 7 6. Main Cntrller 9 7. Operatin Befre Operatin Preparatin f Material Getting Started Hw t set Temperature Hw t set time Start Sterilizatin Finishing Sterilizatin Aut-tuning Service Manual Draining and refilling water Spills Changing water Electric cntrl panel Electric Prblem Pressure pipe lines Setting Parameters Parameter Parameter Inspectin Lg Service Reprt 23
3 CHAPTER 1. BEFORE USE:- Thank yu fr chsing Labnics Prducts Please read this peratin manual carefully befre use fr yur safety and ptimum perating perfrmance. If yu have any questin, please cntact sales representative r service department. This manual cntains imprtant perating and safety infrmatin. Yu must carefully read and understand the cntents f this manual prir t the use f this equipment. CHAPTER 2. SAFETY PRECAUTION:- Warning:- Warning alert yu t a pssibility f persnal injury Cautin:- Cautin alerts yu t a pssibility f damage t the equipment. Dear First Time User, Yur Autclave/Sterilizer using high temperature steam and high pressure. If yu are nt trained fr Autclave usage r nt familiar with Autclave, please d nt perate befre yu are trained r cmpletely read this manual and understand abut yur Autclave fr yur health and safety and t prevent persnal injury. If yu d nt understand, please cntact yur labratry supervisr r technician fr further training curse r advise. LABNICS IS NOT RESPONSIBLE FOR ANY DAMAGE OF PROPERTY OR PERSONAL INJURY FOR ABUSE OR USAGE FOR WRONG PURPOSE OF AUTOCLAVE 2.1 Safety Autclaves are such a familiar feature in many labratries that it is easy t frget what hazards they can pse. The autclave's jb is t render its cntents sterile, r free f any living rganisms. If it fails t d s, serius health 2 hazards can result. The ht, pressurized steam (121 C, 1.2 kg/cm in gauge) that autclaves generate t d this jb makes them serius burn hazards as well. And, because cnditins created inside steam autclaves are s extreme, autclaves can easily malfunctin if they are nt carefully. Befre using Labnics Prduct. Labnics autclave fr the first time read and thrughly understands the wner's manual. If yu cannt lcate the manual, cntact lcal distributr r us directly t get a cpy
4 KEEP WARNING FOR YOUR SAFETY DO NOT OPERATE WITHOUT WATER INSIDE OF THE CHAMBER HEATER IN THE CHAMBER SHOULD BE IMMERSED IN THE WATER TO PREVENT OVER HEATING OR MALFUNCTION IF THERE IS NO WATER IN THE CHAMBER DURING OPERATION THE AUTOCLAVE WILL MOMENTARILY OVERHEAT. THIS MAY CAUSE MALFUNCTION OF AUTOCLAVE HOT DURING OPERATION, ANY PART OF THE AUTOCLAVE TOUCHING THE TOP OF THE AUTOCLAVE OR COVER MAY RESULT IN SEVERE BURNING. - cause by cntact with the utside f autclave - caused by cntact when remving autclaved items - caused by cntact with pressurized steam HOT KEEP AWAY FROM AUTOCLAVE AT LEAST 50 cm during OPERATION TO PREVENT BURNING CAUSED BY LEAKAGE OF STEAM. WARNING - BURN HAZARDS CLOGGING LINES, EQUIPMENT MALFUNCTION OR FAILURE IN THE STEAM SUPPLY MAY CAUSE THE AUTOCLAVE CHAMBER TO FILL WITH SCALDING WATER. IF THE WATER LEAKS FROM ANY PART OF THE AUTOCLAVE, DO NOT OPEN THE CHAMBER DOOR. BURNS FROM SCALDING WATER MAY OTHERWISE RESULT. POTENTIAL EXPOSURE TO INFECTIOUS AGENTS THROUGH EITHER AIRBORNE, DROPLET OR CONTACT TRANSMISSION WHEN PROCESSING LIQUIDS, USE ONLY FLASKS AND SELF-VENTING AUTOMATIC SEALING STOPPERS RECOMMENDED BY THE MANUFACTURER. THIS WILL HELP TO PREVENT SEALED FROM EXPLODING AND LIQUIDS IN GLASS CONTAINERS FROM RUPTURING. WASTE THAT CONTAINS BLEACH MAY HARM AN AUTOCLAVE CAREFULLY PREPARE ITEMS FOR AUTOCLAVING. LOOSELY COVER OR CAP CONTAINERS TO AVOID OVER-PRESSURIZATION KEEP LOADS SMALL - OVERLOADING HINDER STEAM PENETRATION
5 BAGS SHOULD BE OPEN AND SHOULD BE CONTAINED WITHIN THE STAINLESS STEEL TRAY OR BASKET. HOT HOT AFTER COMPLETELY VENTILATED, OPEN CHAMBER DOOR ABOUT ONE CENTIMETER AND ALLOW TEN MINUTES TO LET THE LOAD COOL BEFORE REMOVING TRAY. ALL STEEL PARTS OF AUTOCLAVE ARE HOT. DO NOT TOUCH DURING OPERATION OR STERILIZATION CYCLE. NEVER AUTOCLAVE NITROCELLULOSE TUBES - THEY CAN EXPLODE! DO NOT PUT FLAMMABLE, VOLATILE OR EXPLOSIVE MATERIALS INSIDE OF THE CHAMBER DO NOT REMOVE THE GROUND PRONG (THIRD PIN) (IN CASE OF 110V) FROM THE POWER CORD, OR USE AN UNGROUNDED ADAPTER. THE AUTOCLAVE REQUIRES A 3- WIRE RECEPTACLE. IF ONE IS NOT AVAILABLE, CONSULT WITH AN ELECTRICIAN FOR INSTALLATION. THE MAIN VOLTAGE MUST CORRESPOND TO THE VOLTAGE GIVEN ON THE NAME PLATE. YOUR AUTOCLAVES ARE 120VAC 60HZ OR 220VAC 50/60HZ SINGLE PHASE TO REDUCE THE RISK OF ELECTRICAL SHOCK, DO NOT OPEN THE BODY. NO USER SERVICEABLE PARTS INSIDE. PLACE AUTOCLAVE ON THE FLAT AND LEVELED SURFACE BE SURE TO CONNECT DRAIN AND STEAM VENTILATION VALVE TO DRAIN SINK USE DISTILLED WATER ONLY TO PROTECT HEATER AND SENSOR CHANGE WATER IN THE CHAMBER FREQUENTLY TO PREVENT CONTAMINATION AND ODOR. USING AUTOCLAVE DEODORANT RECOMMENDED DO NOT OPEN THE CHAMBER DOOR AT ANY TIME UNTIL THE PRESSURE GAUGE INDICATING ZERO (0) OPEN "MANUAL SAFETY PRESSURE RELEASE VALVE" ENTIRELY EVERY TIME OPEN THE CHAMBER DOOR AFTER OPERATION - 3 -
6 2.2 Pwer Cnnectin Cautin 1. Yur Autclave is designed fr 110VAC 60Hz 1P r 220VAC 50 / 60Hz Single Phase. 2. Check electrical requirement n the name plate befre use. Name plate is lcated in electric crd cnnectin. Mdel Electrical Requirement Amp Phase LAC ~240 VAC 50/60Hz 3 kw 14 AMP Single Phase 3. Cnnect t receptacle with grund cnnectin. 4. Make sure t supply enugh electrical current. 5. The pwder scket and plug fr LAC-60 is 220VAC SINGLE PHASE. DO NOT cnnect t 380VAC THREE PHASE. 2.3 Installatin Cautin 1. Install autclave where water supply and water drainage is pssible. Yur autclave using water fr steam sterilizatin, it is highly recmmended t install n a place where water supply and water drainage is cnvenient. 2. D nt use in high humid envirnment r install beside delicate instruments. Yur autclave generates heat and steam during peratin, it may damage ther delicate instruments. 3. Install autclave ven at least 100mm apart frm side and back wall. Steam and drain water frm the back f autclave. 4. Place flat, rigid and leveled surface. 5. When mving autclave d nt up-side-dwn. 6. D nt place any bject n the tp f autclave. 2.4 Operatin Warning 1. Ht surface may cause serius injury. Always wear prtect gear during peratin. D nt cntact surface f autclave especially hatch dr withut prtective gear. Ht surface may cause serius injury. D nt pen dr during peratin when pressure engaged in the chamber Be careful ht steam when yu pen hatch dr after sterilizatin cycle. 2. D nt put vlatile, flammable and explsive material inside f autclave 3. D nt put vlatile, flammable and explsive material nearby autclave
7 Cautin 1. D nt perate withut water in the autclave chamber. 2. Be careful nt t spill water t cntrl panel. 3. Use distilled water r deinized water. 2.5 Maintenance Cautin 1. D nt pur water r any liquid when yu clean autclave 2. D nt use highly rganic slvent fr cleaning surface f autclave 3. Drain water and refill fresh water in the chamber after sterilizatin cycle t prevent cntaminatin. CHAPTER 3. FEATURE & ADVANTAGE LABNICS AUTOCLAVE IS... 2 Fr sterilizatin f bacteria, micrrganism and virus with steamed ht air usually at 121 C, 1.2 kgf/cm. Sterilizatin fr labratry ware needs being sterilized befre use such as flasks, bttles, pipets animal cages and plastic ware, brth r agar media fr bacterial culture r dispsal f bihazard wastes such as syringe, clth, glves r Petri dishes. Feature : 1. Vertical lading with service basket. 2. Stainless Steel 304 (AISI304) interir, lid and tp t prtect frm crrsin by water and steam. 3. Incly heating element, prvides lng life, resistant t crrsin and high temperature in repeated use. 4. Manual air purge and bleed valve 5. Autmatic air inlet purge valve 6. Mechanical ver pressure safety valve prevents the pressure surpassing the set maximum limit. 7. Over-temperature security discnnects the heating element in the event f ver temperature r ver pressure surpassing the set maximum limit. 8. Over temperature thermstat discnnects pwer t the heating element in the event f ver temperature r the lack f water in the reservir. 9. Electric circuit breaker fr electrical safety. 10. Digital PID cntrller precisely cntrls the set temperature within ±0.5 C and sterilizatin time in min:sec. 11. Set temperature, time and pressing start buttn autmatically finish sterilizatin cycle and alarm end signal when sterilizatin cycle is finished. 12. Dual LED displays current temperature and set temperature r remaining time simultaneusly. 13. Pressure gauge 14. Equipped with drain valve fr cnvenience. 15. Adjustable needle valve which can cntrl exhaust pressure depending n it's applicatin
8 Specificatins FRAME SPCC metallic bdy with heavy duty epxy pwder cating in white and green 10mm thick SPCC metallic tp plate t be assembled with chamber, hinge, latch and dr Stainless Steel 304 (AISI304) tp cver Fur casters with stpper INTERNAL CHAMBER Stainless Steel 304 Plished (AISI 304) 3 mm thick. Stainless Steel dr with silicne rubber packing. Incly heater. Drain valve. CONTROL SYSTEM Digital PID micrprcessr cntrl system Aut-tuning PV & SV Dual 4-Digit LED Digital Display Wait-Off Timer : 59min59sec / cntinuus Autmatic sterilizatin cycle SAFETY SYSTEM Pressure Gauge Mechanical ver pressure safety valve prevents the pressure surpassing the set maximum limit. Over-temperature security discnnects the heating element in the event f ver temperature r ver pressure surpassing the set maximum limit. Over temperature thermstat discnnects pwer t the heating element in the event f ver temperature r the lack f water in the reservir. Electric circuit breaker fr electrical safety. ALARM SYSTEM Audible and visual alarm system fr ver temperature and ver pressure. Standard Accessries Perfrated Basket : 2 EA Instructin Manual : 1 EA - 6 -
9 CHAPTER 4. SPECIFICATIONS Mdel LAC-60 Dimensins Inner φ350 x 620H (W x D x H) mm Outer 670 x 510 x 990 Capacity 60 l Heater 3.0 kw Temperature Range Ambient + 5 C t 123 C Accuracy Unifrmity Display Cntrller Timer CHAPTER 5. PARTS AND FUNCTIONS ±0.5 C at 121 C ±2.0 C at 121 C PV & SV Dual Digital Display PID Multi-Functin Cntrller 59min59sec / cntinuus Material Inner Stainless Steel (SUS304) Outer Pwder Cated Steel Operating Pressure 1.2 kg/cm Safety Over Heat Prtectr Over Current & Leakage Breaker Electric Supply 110V, 60Hz r 220 V, 50/60 Hz Dr handle Circuit Breaker Pwer Switch Pressure Gauge Circuit breaker Main cntrller Main Pwer Switch Safety Pressure Relief Valve Pressure Guage Hatch dr Caster - 7 -
10 MAIN POWER SWITCH Turn electrical pwer n. PRESSURE GAUGE 2 Gauge indicates pressure in the chamber. Usual maximum perating pressure is 1.25 kgf/cm. Make sure the pressure gauge indicates 0 (zer) every time befre pening dr. Pressure Range and Characteristics Pressure 0 ~ 1.2 kgf/cm 1.2 ~ 1.4 kgf/cm > 1.4 kgf/cm Range Preheating Pressure Sterilizatin Pressure Danger Clr Yellw Zne Green Zne Red Zne Characteristic within this range, within this range, chamber Over this range steam can autclave preheating t temperature is 121~124 C be leak and explde arund increase pressure and fr sterilizatin. the chamber dr temperature t enter sterilizatin cycle Actin N sterilizatin Sterilizatin Press STOP t stp heating Press STOP t vent ut steam Open Manual Safety slwly 2 What t d when pressure increase ver > 1.4 kgf/cm (Dangerus Range) 1) Press STOP Buttn t cut ff heating and venting ut pressure frm the chamber 2) Open Manual Safety Pressure Relief Valve Slwly. If yu pen valve quickly, pressure suddenly increase t explde 3) DO NOT turn ff MAIN POWER SWITCH that will clse SOLENOID VALVE t prevent ver pressure venting 4) 2 Leave Autclave until the pressure dwn t 0 kgf/cm 5) Leave Autclave t cl dwn t ambient temperature 6) Cntact yur lcal technical service engineer MANUAL SAFETY PRESSURE RELEASE VALVE Open the valve and release pressure t 0 (zer) in the chamber every time pening dr fr user safety. Open slwly. When the pressure in the chamber wuld nt release autmatically, user can lwer pressure in the chamber manually. DOOR HANDLE Turn the handle clckwise tightly t lck the dr befre peratin DOOR Clse dr tightly and n the crrect psitin - 8 -
11 STEAM OUTLET Steam and pressure is releasing t the Drain Outlet after sterilizatin cycle is finished. Avid bdy cntact t prevent injury because ht and high pressure steam is exhausting frm the valve during peratin. Drain Valve Drain Outlet Open Clse Steam Outlet Drain Outlet Drain Valve DRAIN VALVE Open the valve t drain water in the chamber Drain valve is inside f the dr lcated n left side f autclave. D nt pen drain valve when there is pressure engaged in the chamber CHAPTER 6. MAIN CONTROLLER PV TEMP (RPM) SV START STOP MODE ON Start / Stp Set Parameter Shift / Left HEAT DSP [AT] Shift / Right Increment Display Mde Aut-tunning TIMER AT START STOP MODE DSP [AT] - 9 -
12 [DISPLAY] PV (Prcess Value) LED Display Displays current temperature f the chamber SV (Set Value) LED Display Displays user set temperature (SV) r remaining time t finish Press DSP t shw SV temp. and time alternatively. [PILOT LAMPS] ON LAMP Lamp blinks during peratin. HEAT LAMP Lamp glws when heater is n. Lamp blinks during peratin. TIMER LAMP Lamp glws when user input timer value. Lamp blinks when press START until PV reach t SV temperature. Lamp stp blinking and glws when PV reach t SV temperature. AT LAMP Blinks during aut-tuning. [BUTTONS] START/STOP BUTTON Start and Stp peratin. MODE BUTTON Push t change user set values. SHIFT BUTTON Mve cursr t left t change values. SHIFT BUTTON Mve cursr t right t change values. INC BUTTON Change set values by 1 increment. AUTO-TUNE Display set value f time and temperature alternatively. Press and hld t start Aut-Tuning
13 CHAPTER 7. OPERATION 7.1 Befre Operatin 1) The main vltage must crrespnd t the vltage given n the name-plate. 2) Place Autclave n the flat and level surface. 3) Cnnect steam utlet (drain valve) t silicne tubing and extend t drain sink. DO NOT REMOVE THE GROUND PRONG (THIRD PIN) (IN CASE OF 110V) FROM THE POWER CORD, OR USE AN UNGROUNDED ADAPTER. THE AUTOCLAVE REQUIRES A 3-WIRE RECEPTACLE. IF ONE IS NOT AVAILABLE, CONSULT WITH AN ELECTRICIAN FOR INSTALLATION YOUR AUTOCLAVES ARE 120VAC 60HZ OR 220VAC 50/60HZ SINGLE PHASE TO REDUCE THE RISK OF ELECTRICAL SHOCK, DO NOT OPEN THE BODY. NO USER SERVICEABLE PARTS INSIDE 7.2 Preparatin f Material 1) All materials must be autclavable. 2) Vessels are lsely capped. 3) Liquid cntained in a vessel is at least twice the vlume f the liquid t be autclaved. 4) All materials are placed in an autclavable tray with sides at least 4 inches high. All materials autclaved must be placed in an autclavable tray with sides that are at least 10 cm in height. The use f trays shuld eliminate anything biling ver and spilling int the autclave. Therefre, all materials must fit within this tray. Only autclavable materials may be placed in the autclave. If yu are unsure if smething is autclavable, check with the lcal distributr r cntact us directly. Liquids t be autclaved must be in an autclavable vessel that is at least twice as large as the vlume t be autclaved (i.e. If yu are autclaving 1 liter f media, yu need t put it in a flask that hld at least 2 liters). This allws fr biling during the cycle. Caps must be slightly lse s that pressure created during the cycle des nt cause the vessel t break. Fr screw cap cntainers yu can make the lid hand tight and then lsen the lid by ne-half turn. Dry gds must be placed in an autclavable tray with 10 cm sides als. Garbage must be put in autclavable bags and inside the autclavable trays with 10 cm sides. Sharps cntainers must als g int the same type f autclavable trays. T ensure prper sterilizatin f bth garbage and sharps cntainers, 1 liter f water shuld be added t the bags r sharps cntainers. This water will vaprize during the autclave cycle t allw better penetratin f steam and mre efficient sterilizatin. Autclave garbage bags must be tied ff. Warning: Use f nn-autclavable trays and cntainers may result in injury t the user and damage t the equipment
14 WARNING and SAFETY PRECAUTION POTENTIAL EXPOSURE TO INFECTIOUS AGENTS THROUGH EITHER AIRBORNE, DROPLET OR CONTACT TRANSMISSION WHEN PROCESSING LIQUIDS, USE ONLY FLASKS AND SELF-VENTING AUTOMATIC SEALING STOPPERS RECOMMENDED BY THE MANUFACTURER. THIS WILL HELP TO PREVENT SEALED FROM EXPLODING AND LIQUIDS IN GLASS CONTAINERS FROM RUPTURING WASTE THAT CONTAINS BLEACH MAY HARM AN AUTOCLAVE CAREFULLY PREPARE ITEMS FOR AUTOCLAVING. LOOSELY COVER OR CAP CONTAINERS TO AVOID OVER-PRESSURIZATION KEEP LOADS SMALL - OVERLOADING HINDER STEAM PENETRATION. BAGS SHOULD BE OPEN AND SHOULD BE CONTAINED WITHIN THE STAINLESS STEEL TRAY OR BASKET NEVER AUTOCLAVE NITROCELLULOSE TUBES - THEY CAN EXPLODE! DO NOT PUT FLAMMABLE, VOLATILE OR EXPLOSIVE MATERIALS INSIDE OF THE CHAMBER 7.3 Getting Started 1) Clse Drain Valve t lck. Turn handle crss t pipeline. Drain valve is lcated in the right side panel. 2) Pur distilled water in the chamber t level f just beneath the heater prtectin cver is just immersed in the water. * Heater shuld be immersed in water at any time 3) If water level is lw LED displays alarm FILL when yu press START buttn 4) Put prepared materials int prvided basket and put basket int the autclave chamber. 5) Clse chamber dr in right psitin and fasten dr handle tightly t clckwise. 6) Clse Manual Safety Pressure Release Valve in frnt f autclave t clckwise tightly. 7) Turn circuit breaker n which lcated n the back panel. 8) Turn main pwer switch n lcated n the left side f the main cntrller. PV LED displays current temperature f the chamber. SV LED displays user set temperature (Factry default value is 121 C). Press AUTO-TUNE buttn t display time t autclave and temperature alternatively. (Factry default value is minutes)
15 WARNING DO NOT OPERATE WITHOUT WATER INSIDE OF THE CHAMBER HEATER IN THE CHAMBER SHOULD BE IMMERSED IN THE WATER TO PREVENT OVER HEATING OR MALFUNCTION IF THERE IS NO WATER IN THE CHAMBER DURING OPERATION THE AUTOCLAVE WILL MOMENTARILY OVERHEAT. THIS MAY CAUSE MALFUNCTION OF AUTOCLAVE 7.4 Hw t set Temperature Press MODE buttn t set the temperature. LED display panel n tp (PV) displays 'TEMP' LED display panel n bttm (SV), last digit f the LED display blink and prmpt user input. (000) Press 3buttn t mve t digit yu want change value. Press 5buttn t change value frm 0 t 9. Set three digits f temperature value yu want t perate. Factry default temperature is 121 C The maximum perating temperature is nt allwed ver 121 C 7.5 Hw t set Time Press MODE buttn after temperature set finished. LED display panel n tp (PV) displays 'Time' LED display panel n bttm (SV), last digit f the LED display blink and prmpt user input. (00.00 in mm:ss) Press 3buttn t mve t digit yu want change value. Press 5buttn t change value frm 0 t 9. Set fur digits f time yu want t perate. Yu can set timer up t 59 min 59 sec maximum. (Factry default time value is 20:00 min.) Press MODE buttn t finish timer setting 7.6 Start Sterilizatin After set temperature and time, PV LED displays current temperature f the chamber and SV LED displays time r perating temperature. Be sure all safety prcedure is finished. Press START buttn t start peratin. TIMER LAMP blinks until the current chamber temperature reaches t the user set value. When the current chamber temperature reaches t the user set value the TIMER LAMP stps blinking and starts cunt dwn. Air in the chamber is releasing t Steam Outlet until the chamber temperature reach t 105 C. At this pint Electrnic Slenid Valve autmatically lcked t increase pressure in the chamber. Refer ALL Parameter Setting t change the temperature t lck slenid valve. Temperature cntinuusly increase and stabilize until t reach 121 C. When the temperature stabilized at 121 C, timer starts cunt dwn
16 The autclave will cntinue t sterilizatin cycle until the time selected runs ut. The autclave CANNOT be pened at any reasn until the chamber pressure will slwly drp alng with the temperature. If yu want t stp sterilizatin, press STOP buttn. Autclave autmatically stp and slenid valve is pen t release pressure in the chamber. DO NOT pen the chamber dr until the pressure drp t 0 When sterilizatin time is run ut, Electrnic Slenid Valve autmatically pened t release pressure in the autclave chamber slwly. When chamber temperature cl dwn t 95 C, SV LED displays END and beeps 30 secnds. Wait until the Pressure Gauge indicates 0 (zer) D nt pen chamber dr immediately after finishing sterilizatin r END signal. TEMP PRESSURE kg/cm 121 C Alarm Limit High (ALH) 105 C Alarm Limit Lw (ALL) 2 0 kg/cm AMB. TEMP. 95 C (AOFF) START CLOSE SOLENOID VALVE TIMER RUNS UP OPEN SOLENOID VALVE SAFETY PRECAUTION HOT TIMER START COUNT DOWN END SIGNAL BEEP DURING OPERATION, ANY PART OF THE AUTOCLAVE TOUCHING THE TOP OF THE AUTOCLAVE OR COVER MAY RESULT IN SEVERE BURNING. - cause by cntact with the utside f autclave - caused by cntact when remving autclaved items - caused by cntact with pressurized steam HOT KEEP AWAY FROM AUTOCLAVE AT LEAST 50 cm during OPERATION TO PREVENT BURNING CAUSED BY LEAKAGE OF STEAM. WARNING - BURN HAZARDS CLOGGING LINES, EQUIPMENT MALFUNCTION OR FAILURE IN THE STEAM SUPPLY MAY CAUSE THE AUTOCLAVE CHAMBER TO FILL WITH SCALDING WATER. IF THE WATER LEAKS FROM ANY PART OF THE AUTOCLAVE, DO NOT OPEN THE CHAMBER DOOR. BURNS FROM SCALDING WATER MAY OTHERWISE RESULT
17 7.7 Finishing Sterilizatin SAFETY PRECAUTION Wear a lab cat, eye prtectin, heat resistant glves and clsed te shes. Once the sterilizatin cycle is cmpleted, the LED indicates END signal and beep sund fr 30 secnds and yur autclave finishing sterilizatin cycle. Check the Pressure Gauge indicating 0 (zer) D nt pen chamber dr immediately after finishing sterilizatin. Be sure t wear safety gggle and heat prtectin glves t avid direct cntact t prevent burning. Turn the MANUAL SAFETY PRESSURE RELEASE VALVE n the frnt t cunter clckwise t release pressure which may remain in the chamber fr yur safety. After cmpletely ventilated, pen the chamber dr abut ne centimeter and allw ten minutes t let the lad cl befre remving tray r basket. Open the dr and retrieve autclaved materials. HOT HOT POTENTIAL EXPOSURE TO INFECTIOUS AGENTS THROUGH EITHER AIRBORNE, DROPLET OR CONTACT TRANSMISSION AFTER COMPLETELY VENTILATED, OPEN CHAMBER DOOR ABOUT ONE CENTIMETER AND ALLOW TEN MINUTES TO LET THE LOAD COOL BEFORE REMOVING TRAY. AUTOCLAVED ITEMS ARE HOT WHEN RETRIEVING FROM THE AUTOCLAVE CHAMBER. BE SURE TO WEAR PROTECTION GLOVES AND GOGGLES. DO NOT start anther sterilizatin cycle befre temperature drp dwn t 30 C. It may cause ver 2 pressure ver 1.4 kgf/cm befre temperature reach t 121 C. 7.8 Aut-tuning AT (Aut-Tuning) functin prvides the autclave t reach the required set temperature fast and accurately. Yur autclave was aut-tuned and tested befre shipment. User d nt need t aut-tune. If parts listed belw was replaced r serviced, reset all parameters and aut-tune again. Replacement Parts t be aut-tune again ; 1. PID Cntrller 2. Heater 3. PT Sensr
18 Aut-Tuning Prcedure ; 1. Prcess sterilizatin prcedure same as described befre. 2. Empty autclave chamber 3. Set temperature at 121 C 4. Set time t Press START BUTTON 6. Press and hld AUTO-TUNE buttn fr 5 secnds 7. Aut-tuning starts with beep sund 8. AT INDICATION Lamp start blinks during aut-tuning 9. Aut-Tuning usually takes 30 minutes 10. Aut-tuning finish with beep sund and AT INDICATION LAMP STOP blinking. 11. Turn ff Autclave by pressing START/STOP buttn. 12. Wait until the chamber temperature drp dwn t belw. 13. Turn the MANUAL SAFETY PRESSURE RELEASE VALVE n the frnt t cunter clckwise t release pressure which may remain in the chamber fr yur safety. 14. If the pressure in the chamber cmpletely ventilated pen the chamber dr and turn ff the Main Pwer Switch. 15. Yur Autclave is aut-tuned. CHAPTER 8. SERVICE MANUAL 8.1 Draining and Refilling Water Drain Valve Open Drain Outlet Clse Steam Outlet Drain Valve Drain Outlet Befre draining, yur autclave shuld be in ambient temperature and atmsphere pressure and chamber dr shuld pened. Open the cabinet dr n the right side f the autclave Be sure t the drain utlet is cnnected t drain sink r waste basket Open the Drain valve t pen psitin (parallel t thepipe line) Drain water in the chamber cmpletely. If necessary clean the chamber Clse the Drain valve again Refill the chamber with distilled water t slightly immerse heater cver n the bttm f the chamber. DO NOT pen drain valve when pressure engaged in the chamber 8.2 Spills If there is a spill inside the autclave chamber, allw the unit t cl befre attempting t clean up the spill. If glass breaks in the autclave, use tngs, frceps r ther mechanical means t recver fragments. D nt use bare r glved hands t pick up brken glassware. If a spill ccurs in the autclave rm, please cntact yur labratry safety manager 8.3 Changing Water T prevent cntaminatin and dr, change water at least every five autclave cycle
19 8.4 Electric Cntrl Panel TRIAC Cntrller Over Temperature Safety Magnet S/W Circuit Breaker Relay Mechanical Pressure Safety Valve Nise Filter Needle Valve Slenid Valve Drain Valve Over Temperature Safety : Mechanical ver temperature cut ff device t prtect ver temperature f cntrller malfunctin. Set at just abve 123 C Relay : If relay light is n, slenid valve is pen Needle Valve : Cntrls pressure release time. Slenid Valve : Autmatically pen and clse during sterilizatin cycle. Drain Valve : Drain ut water in the chamber Mechanical Pressure Safety Valve : Mechanically release ver pressure fr safety
20 8.5 Electrical Prblem Errr Display Cause Check Slutin N Display NONE N main pwer Check pwer supply Plug firmly cnnectin cable Pwer failure Check main circuit Turn circuit breaker n breaker Circuit breaker trip NONE Lw electrical pwer Check pwer Supply enugh pwer supply cnsumptin f supply autclave Electric Leakage r Check heater with Replace heater shrtage f heater multi-meter Heating Failure NONE Lw Over Temperature Over Temperature Set Over Temperature Prtectin Setting prtectin in the cabinet Prtectin dial higher than 140 C Heater discnnectin Check heater with Replace heater multimeter Sensr prblem NNNN Shrtage f PT Sensr Check PT sensr integrity Replace PT Sensr uuuu Open PT Sensr Check PT sensr integrity Replace PT Sensr Over Temperature uuuu Cntrller malfunctin Check Cntrller Replace Cntrller higher than 125 C uuuu Shrtage f TRIAC Check TRIAC Replace TRIAC uuuu Shrtage f circuit Check circuit and electric Replace defective parts cmpnents Lw Water Level FILL Lw water level Check water level Fill water up t water level sensr 8.6 Pressure Pipe Lines Leakage n the pressure pipe line may cause severe damage f the autclave r safety prblem. Call authrized service engineer if yu fund any leakage f steam r water drplets thrugh the pipe line. CHAPTER 9. SETTING PARAMETER Imprtant parameters are lcked befre shipment fr yur safety. This sectin is fr service technician nt fr Autclave user. D nt change imprtant parameters described belw at any time. Factry default values are different frm prduct t prduct. Every individual Autclaves are tested and adjusted at it's ptimum parameter setting. Parameter value shwn belw are just fr reference
21 9.1 Parameter 1 T set parameters, Get back t nrmal display mde Press and hld MODE Buttn fr 5 secnds. LED displays "ALH" and waiting fr user input. Press SHIFT and INC Buttn t change values. Press MODE Buttn t g next parameter. T escape frm Parameter mde t nrmal display mde, press and hld MODE Buttn fr 6 secnds. Parameter Name f Parameter Setting Range Factry User Set Value Symbl and Descriptins Default Value ALH ALARM LIMIT HIGH 00.0 ~ DO NOT CHANGE (RELATIVE VALUE) Relative value f temperature f High Alarm where cntrller stp utput t heater. Ex. If SV is 121 C and ALH is 2 C, cntrller stp utput t heater when the PV is higher than 123 C. ALL ALARM LIMIT LOW 00.0 ~ DO NOT CHANGE (RELATIVE VALUE) Relative value f temperature f Lw Alarm where cntrller clse Slenid Valve during peratin. Ex. If SV is 121 C and ALL is 16 C, cntrller clse Slenid Valve at 105 C t increase temperature and pressure. HYS HYSTERESIS 0.3 DO NOT CHANGE Range f Alarm pint where alarm activate and inactivate. BEEP BEEP ON TIME 0 ~ 9999 SEC 30 DO NOT CHANGE Beep n time in secnds after timer run ut. If the value is set at 0, cntinuusly beep ADJ Temperature Adjustment ~ DO NOT CHANGE Cmpensate temperature deviatin If the actual temperature measured by standard thermmeter is different frm temperature which cntrller read, user can cnpensate temperature difference by Adj functin Ex. Actual temp = Displayed temp = 99.9 Set Adj at 0.1 Actual temp = 99.5 Displayed temp = Set Adj at Lc LOCK PASSWORD 0000, LOCKED Prtect set values and parameters frm unauthrized change N3 N2 N1 N0 Available value t set 0 r 1 0 r 1 0 r 1 0 r 1 Where N3 : KEY LOCK 1 : LOCK 0 : UNLOCK N2 : RESERVED 1 : 0 : N1 : PARAMETER DATA LOCK 1 : LOCK 0 : UNLOCK N0 : SET VALUE DATA LOCK 1 : LOCK 0 : UNLOCK N3 (KEY LOCK) : Prtect pressing buttn. N1 (PARAMETER DATA LOCK) : Prtect parameter values stred in the cntrller N0 (SET VALUE DATA LOCK) : Prtect user set values such as temperature and time
22 9.2 Parameter 2 T set factry parameters, Get back t nrmal display mde Press and hld MODE Buttn fr 30 secnds. LED displays RNT and waiting fr user input. Press SHIFT and INC Buttn t change values. Press MODE Buttn t g next parameter. T escape frm Parameter mde t nrmal display mde, press and hld MODE Buttn fr 6 secnds. Parameter Name f Parameter Setting Range Factry User Set Value Symbl and Descriptins Default RNT Maximum temperature limit t set ~ C 123 DO NOT CHANGE Maximum temperature limit t set. If rnt value set at 125 C, user cannt input perating temperature higher than 125 C If PV temperature is higher than rnt value by any reasn, cntrller displays uuuu and stp peratin. ACTP Temperature where timer activated 00.0 ~ 99.9 C 0 DO NOT CHANGE Abslute value (Current Temp. - Set Temp) > ACTP then timer starts Temp set temp. Temp (Set temp - ACTP) set temp. timer start timer end time timer start timer end time Where ACTP = 0 Where ACTP > 0 PRO Perid 1 ~ 99 sec. 5 DO NOT CHANGE (Output Interval) P Prprtin 0 ~ 9999 Aut-Tune DO NOT CHANGE I Integral 0 ~ 9999 Aut-Tune DO NOT CHANGE D Differential 0 ~ 9999 Aut-Tune DO NOT CHANGE Mde0 OPERATING MODE CONTROL 0000 ~ DO NOT CHANGE N3 N2 N1 N0 Available value t set 0 r 1 0 r 1 0 r 1 0 r 1 Where N3 : TYPE OF SENSOR 1 : DIN Pt 100 hm 0 : KS, JIS Pt 100 hm N2 : ALARM HIGH DATA TYPE 1 : ABSOLUTE 0 : RELATIVE N1 : ALARM LOW DATA TYPE 1 : ABSOLUTE 0 : RELATIVE N0 : DECIMAL PLACE DISPLAY 1 : YES 0 : NO
23 Mde1 OPERATING MODE CONTROL 0000 ~ DO NOT CHANGE N3 N2 N1 N0 Available value t set 0 r 1 0 r 1 0 r 1 r 2 0 r 1 Where N3 : TYPE OF AUTOCLAVE 1 : TYPE A 0 : TYPE B 2 : TYPE C N2 : TIMER FUNCTION 1 : AVAILABLE 0 : NOT AVAILABLE (If the value set at 0, timer setting mde is nt displayed in the cntrller) N1 : TIME SCALE N0 : POWER ON RESTORE 1 : ON 0 : OFF 2 : DD:HH (00.00 ~ 99 days 23 hurs) 1 : HH:MM (00.00 ~ 99 hurs 59 min) 0 : MM:SS (00.00 ~ 99 min 59 sec) (During peratin, if the electrical supply is turn ut and get back again, restre the last perating cnditin and resume perating when POWER ON RESTORE functin is ON) Mde2 OPERATING MODE CONTROL 0000~ DO NOT CHANGE N3 N2 N1 N0 Available value t set 0 r 1 0 r 1 0 r 1 r 2 0 r 1 Where N3 : RESERVED N2 : RESERVED N1 : ALH RELAY N0 : ALARM REPLAY DRAN Fix temperature display within the dran 0 DO NOT CHANGE range AOFF After sterilizatin cycle, temperature where ~ C 95 DO NOT CHANGE cntrller alarm END signal
24 CHAPTER 10. INSPECTION LOG FOR AUTOCLAVE / STERILIZER Mdel # : Serial # : Client : Date & Time: Amb. Temp. : Electricity : VAC Hz LABNICS Pretest check list (Initial after each Checkpint) If Nn-Applicable enter N/A Terminatin f each electrical cnnectins Cntrl panel key switch tight Surge prtectin terminatin N gaps in packing Equipped with prper accessries Esthetics k LABNICS TEST AREA, TEST LOG (NOTE) If units fails any prtin f the test enter "NG" in the blank space adjacent t that check pint Technician Esthetics OK Chassis Pwder Cating Chamber Cntrl panel key switch tight Assembly Heater Pt Sensr OPT Sensr Pressure Gauge Fans Crrect Blade, N Cntact Chamber Dr seal at pull dwn Circuit and Wiring Crrespnds t Circuit Diagram Operatin Unusual nise at startup N excessive dummy panel vibratin Leakage n the pipe line Verify slenid valve Verify ver temp. prtectin Verify ver pressure prtectin valve Unusual vibratin during run Unusual nise during run Unusual fan nise r vibratin Display indicatin and warning functins Temperature Max. 123 C Pressure Max 1.3 Kg/cm Factry Default Setting Temperature 121 C 2 Terminatin f each electrical cnnectins Pressure 1.2 kg/cm Surge prtectin terminatin Sterilizatin Time 20 Min. Mlded plug nt verheated Labeling n cmpnents crrect Electrical Insulatin@500VDC less than 20MΩ Test Run Cycles Hi-Pt 1000VAC, 10mA, 10 sec OK Operatin Manual Included Safety Mark Attached Equipped with prper accessries
25 SERVICE REPORT Custmer s Address : Cntact Persn / Designatin : Tel.N.: Fax N.: Weekly Off.: Dept.: Date Frm Time T System Cnfiguratin Mdel Serial N. Date : Status : OK SR. N. Nt OK Installatin Warranty Demnstratin Maintenance Repairs Cntract Applicatin Calibratin Billable Validatin Curtesy Nature f Prblem : Observatin & Actin Taken : Custmer s Remarks : Parts Replaced : Parts Recmmended / Actin Required : Yes Service Engineer s Name & Signature N Requisitin Number : Custmer s Name, Signature, Date & Stamp Page Of
26 Labnics Equipment Christy St., Fremnt, CA USA. Tll Free : (877) Tel. : (925) Fax : (925) inf@labnics.cm Website :
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